cefepime  (Chem Impex International)

Journal: Nature Communications

Article Title: Cancer cell-secreted IGF2 instigates fibroblasts and bone marrow-derived vascular progenitor cells to promote cancer progression

doi: 10.1038/ncomms14399

Figure Lengend Snippet: ( a ) Invasion ability of ESCC cells induced by the conditioned medium of lung cells from mice bearing the tumours expressing Id1 alone, Id1-shIGF2, or vector control, respectively (scale bar, 100 μm). ( b ) Cytokine profiling of conditioned medium of lung cells from nude mice bearing Id1-expressing and control tumour xenograft, respectively, was performed using a mouse cytokine antibody array. Blue frames indicate the internal positive control and red frames indicate the proteins with markedly increased expression. ( c ) Quantification of CXCL5 level in conditioned medium of lung cells. ( d ) The invasion of KYSE270 cells attracted by CXCL5 in the absence or presence of CXCR2 antibody was determined. ( e ) The invasion ability of KYSE270 cells attracted by conditioned medium from mouse lung cells in the presence or absence of CXCR2 or CXCL5 antibody was compared. Three biological replicates were performed for in vitro assays. Bars, s.d.; * P <0.05; ** P <0.01, *** P <0.001 by Student's t -test.

Article Snippet: The primary antibodies used included VEGF (#sc-152; 1:500 dilution), Id1 (#sc-488; 1:1000 dilution), Id2 (#sc-489; 1:1000 dilution), Id3 (#sc-490; 1:1000 dilution), Id4 (#sc-13047; 1:1000 dilution), PIGF (#sc-1880; 1:1000 dilution, FGF (sc-79; 1:1000 dilution) and actin (#sc-1616; 1:2000 dilution) from Santa Cruz Biotechnology; phospho-MDM2 (#3521; 1:1000 dilution), phospho-AKT (#9271; 1:500 dilution) AKT (#4691; 1:1000 dilution), p53 (#2524; 1:2000 dilution) from Cell Signaling Technology; IGF2 (#AF-292-NA;1:500 dilution) and CXCL5 (#DS-PB-01247; 1:1000 dilution) from RayBiotech; and α-SMA (#A5228; 1:1000 dilution; Sigma).

Techniques: Expressing, Plasmid Preparation, Ab Array, Positive Control, In Vitro